Drug-drug interaction is a public health problem that constitutes one of the leading causes The objective of the present study was to evaluate the in-vitro antimicrobial, antioxidant, and total polyphenolic, flavonoids, and tannin contents of crude extracts of Portulaca olerace. Extracts from each plant were prepared by sequential maceration of dried whole plant powder in solvents of increasing polarity. The antimicrobial activity was evaluated against Gram-positive and Gram-negative, as well as two fungi, by the disc diffusion method. Antioxidant activity was assessed based on the scavenging activity of the stable 2,2-diphenyl-1-picrylhydrazyl free radical (DPPH). Total polyphenolic, flavonoids, and tannin contents were determined by spectrophotometric assays. Generally, the results of antimicrobial activity showed that extracts of the plant exhibited better antifungal activity than antibacterial activity. The highest antifungal activity against Candida albicans was recorded from the methanolic extract of P. olerace (inhibition zone (IZ) =19mm) flowed by ethyl acetate extract (IZ =18mm). While the highest antibacterial activity of the methanolic extract against Pseudomonas aeruginosa was obtained from the disc diffusion method (IZ = 20 mm), The highest scavenging radical activity was obtained from the methanol extract (45%). Quantitative analysis revealed total polyphenols had the highest value shown in methanolic extract (114.17±0.15mg gallic acid equivalents/g). The total flavonoid content was the highest value recorded from the ethyl acetate extract (499.08±0.01 mg quercetin equivalents/g). While the total tannin content had the highest value found in methanol extract (532.18±0.13mg tannic acid equivalents/g).

Keywords: Portulaca oleracea, antimicrobial activity, antioxidant activity, total phenolic, total flavonoids……