In-vitro Anthelmintic Activity of Aqueous Extract of Leaves of Cleome Rutidosperma DC. (Capparidaceae) Against Haemonchus Contortus

Abstract
Background: Cleome rutidosperma is annual herb found in different places in Bangladesh. Its leaves used as the treatment for Helminthiasis associated with Haemonchus contortus. This parasite is one of the most pathogenic nematodes and responsible for anemia, edema, and death of cattle’s, mainly during summer in warm, humid climates as like Bangladesh. The aim of the study to investigate the in-vitro anthelmintic activity of aqueous extract C. rutidosperma (AECR) leaves against H. contortusMethods: We performed phytochemical analysis and in-vitro anthelmintic assay to determine the possible phytochemicals, caused by anthelmintic activity against H. contortusResults: In-vitro anthelmintic assay, the AECR at the doses of 25; 50 and 100mg/mL significantly (p< 0.01) and (p< 0.001) paralyzed and caused the death of H. contortus in a dose-dependent manner. Reference standard drug Albendazole (15 mg/mL) exerts almost same effect as AECR. No paralyzed and death observed at the control group when treated with 0.9% normal saline. Conclusion: This data confirmed that the aqueous extract C. rutidosperma has the in-vitro anthelmintic activity of against H. contortusC. rutidosperma may offer an alternative source for the control of gastrointestinal nematodes of cattle’s.